Catalog Number：VQ3187H
Human IL-10 VeriQuant Immunoassay Kit
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96 T
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MSDS
User Guide(a) Two monoclonal antibodies against distinct epitopes are conjugated with oligo probes, then form a sandwich complex with the antigen.
(b) Following antigen recognition by the paired antibodies, the conjugated oligonucleotides are spatially proximate, and generate an amplifiable dsDNA template under the enzymatic action.
(c) qPCR amplification is then performed. CT values correlate inversely with protein concentration, and sample target concentrations are calculated via standard curve.
Color-coded reagents components for easy identification
Kit outer packaging and components packaging
Product Operation Video
Product Details
| Product Name | Human IL-10 VeriQuant Immunoassay Kit | Species | Human |
| Gene Alias | CSIF; TGIF; GVHDS; IL-10; IL10A | Gene ID | 3586 |
| Uniprot ID | P22301 | Protein Alias | Interleukin-10,IL-10,Cytokine synthesis inhibitory factor (CSIF),IL10,IL 10,CSIF |
| Detection Method | Proximity-based Immunoassay | Sensitivity | 0.083pg/mL |
| Assay Instrument | qPCR Instruments | Detection Range | 0.32-5000 pg/mL |
| Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=1~5μL | ||
| Total Assay Time | 2 H | Manual Handling Time | 20 min |
| Shipping Temperature | Ultra-low temperature phase-change materials | Storage Temperature | -20°C |
| No Washing Step | Yes | Tag or Dye | FAM™ |
| Kit Info | The Human IL-10 VeriQuant Immunoassay Kit is designed for the high-sensitivity quantitative measurement of human IL-10 protein in minimal sample volumes. Utilizing our proprietary ReedLytix conjugation system coupled with Proximity Ligation Assay (PLA) technology, this kit integrates the specific antigen recognition of high-affinity antibodies with the exponential signal amplification power of qPCR. The result is a robust next-generation protein quantification platform. With a simple workflow, results can be obtained from sample to answer in just 2 hours. Data exported from your qPCR instrument can be directly analyzed via our online calculation tool at: https://www.reedbiotech.com/list/114.html ①Ultra-Sensitivity: All products achieve femtogram-level sensitivity ②Wide Range: Product detection range spans 6-log dynamic range ③Micro-Volume: Minimum sample size is only 1μL ④Fast: Results in 2 Hours(30 minutes of manual handling),no plate washing ⑤ALL IN ONE: Can replace traditional, high-sensitivity, micro-fast, fast-step, wide-range ELISA kits simultaneously |
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| Target Info | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. | ||