Catalog Number：VQ1074H
Human IL-1β VeriQuant Immunoassay Kit
Copy Product Infomation
Size：
96 T
Price：
Inquiry
Manual
MSDS
User Guide(a) Two monoclonal antibodies against distinct epitopes are conjugated with oligo probes, then form a sandwich complex with the antigen.
(b) Following antigen recognition by the paired antibodies, the conjugated oligonucleotides are spatially proximate, and generate an amplifiable dsDNA template under the enzymatic action.
(c) qPCR amplification is then performed. CT values correlate inversely with protein concentration, and sample target concentrations are calculated via standard curve.
Color-coded reagents components for easy identification
Kit outer packaging and components packaging
Product Operation Video
Product Details
| Product Name | Human IL-1β VeriQuant Immunoassay Kit | Species | Human |
| Gene Alias | IL-1, IL1-BETA, IL1B, IL1beta, IL1F2 | Gene ID | 3553 |
| Uniprot ID | P01584 | Protein Alias | IL1B, IL1-BETA, IL1F2, catabolin, IL1b, IL-1B, IL-1b,Catabolin, IL-1 beta, Hematopoietin 1 (H1), IFN beta inducing factor, il 1b, IL 1β, IL1 B, IL1β, Osteoclast activating factor (OAF), Pro interleukin 1 beta, ILN, Interleukin, Interleukin 1 beta precursor, Interleukin1 beta, Interleukin-1b, LAF, IL1B, interleukin 1 precursor polypeptide, interleukin 1beta, Interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta, unnamed protein product |
| Detection Method | Proximity-based Immunoassay | Sensitivity | 0.077pg/mL |
| Assay Instrument | qPCR Instruments | Detection Range | 0.32-5000 pg/mL |
| Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=1~5μL | ||
| Total Assay Time | 2 H | Manual Handling Time | 20 min |
| Shipping Temperature | Ultra-low temperature phase-change materials | Storage Temperature | -20°C |
| No Washing Step | Yes | Tag or Dye | FAM™ |
| Kit Info | The Human IL-1β VeriQuant Immunoassay Kit is designed for the high-sensitivity quantitative measurement of Human IL-1β protein in minimal sample volumes. Utilizing our proprietary ReedLytix conjugation system coupled with Proximity Ligation Assay (PLA) technology, this kit integrates the specific antigen recognition of high-affinity antibodies with the exponential signal amplification power of qPCR. The result is a robust next-generation protein quantification platform. With a simple workflow, results can be obtained from sample to answer in just 2 hours. Data exported from your qPCR instrument can be directly analyzed via our online calculation tool at: https://www.reedbiotech.com/list/114.html ①Ultra-Sensitivity: All products achieve femtogram-level sensitivity ②Wide Range: Product detection range spans 6-log dynamic range ③Micro-Volume: Minimum sample size is only 1μL ④Fast: Results in 2 Hours(30 minutes of manual handling),no plate washing ⑤ALL IN ONE: Can replace traditional, high-sensitivity, micro-fast, fast-step, wide-range ELISA kits simultaneously |
||
| Target Info | Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value. | ||