Catalog Number：VQ2824M
Mouse IgE VeriQuant Immunoassay Kit
Copy Product Infomation
Size：
96 T
Price：
Inquiry
Manual
MSDS
User Guide(a) Two monoclonal antibodies against distinct epitopes are conjugated with oligo probes, then form a sandwich complex with the antigen.
(b) Following antigen recognition by the paired antibodies, the conjugated oligonucleotides are spatially proximate, and generate an amplifiable dsDNA template under the enzymatic action.
(c) qPCR amplification is then performed. CT values correlate inversely with protein concentration, and sample target concentrations are calculated via standard curve.
Color-coded reagents components for easy identification
Kit outer packaging and components packaging
Product Operation Video
Product Details
| Product Name | Mouse IgE VeriQuant Immunoassay Kit | Species | Mouse |
| Gene Alias | IGHE / IgE / Gm900 / LOC380792 | Gene ID | 380792 |
| Uniprot ID | P06336 | Protein Alias | Mouse IgE |
| Detection Method | Proximity-based Immunoassay | Sensitivity | 0.0368pg/mL |
| Assay Instrument | qPCR Instruments | Detection Range | 0.64-10000pg/mL |
| Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=1~5μL | ||
| Total Assay Time | 2 H | Manual Handling Time | 20 min |
| Shipping Temperature | Ultra-low temperature phase-change materials | Storage Temperature | -20°C |
| No Washing Step | Yes | Tag or Dye | FAM™ |
| Kit Info | N/A | ||
| Target Info | [Note: Novoprotein does not offer a mouse IgE product; the text below is taken from the Human IGHE product (C35D).] Immunoglobulin heavy constant epsilon is knowed as Immunoglobulin (Ig) E, which causes the immediate allergic reaction, is produced by B lymphocytes through the class switching. Constant region of membrane-bound IgE (long mIgE), part of the B cell receptor complex (BCR). Upon antigen cross-linking triggers quick BCR signaling, ensuring survival of IgE-switched B cells and differentiation into plasma cells, thus regulating both primary and memory IgE responses. | ||