Catalog Number：VQ1026H
Human GROα/CXCL1 VeriQuant Immunoassay Kit
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Size：
96 T
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Manual
MSDS
User Guide(a) Two monoclonal antibodies against distinct epitopes are conjugated with oligo probes, then form a sandwich complex with the antigen.
(b) Following antigen recognition by the paired antibodies, the conjugated oligonucleotides are spatially proximate, and generate an amplifiable dsDNA template under the enzymatic action.
(c) qPCR amplification is then performed. CT values correlate inversely with protein concentration, and sample target concentrations are calculated via standard curve.
Color-coded reagents components for easy identification
Kit outer packaging and components packaging
Product Operation Video
Product Details
| Product Name | Human GROα/CXCL1 VeriQuant Immunoassay Kit | Species | Human |
| Gene Alias | CXCL1 / GRO / GRO1 / GROA / MGSA / SCYB1 | Gene ID | 2919 |
| Uniprot ID | P09341 | Protein Alias | Human GROα/CXCL1 |
| Detection Method | Proximity-based Immunoassay | Sensitivity | 0.0965pg/mL |
| Assay Instrument | qPCR Instruments | Detection Range | 1.28-20000pg/mL |
| Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=1~5μL | ||
| Total Assay Time | 2 H | Manual Handling Time | 20 min |
| Shipping Temperature | Ultra-low temperature phase-change materials | Storage Temperature | -20°C |
| No Washing Step | Yes | Tag or Dye | FAM™ |
| Kit Info | N/A | ||
| Target Info | Chemokine (C-X-C motif) Ligand 1 Protein (CXCL1) is a growth factor for melanoma cells and a chemotaxin for neutrophils and a member of the CXC chemokine family that is a potent neutrophil attractant and activator and is also active toward basophils. CXCL1 is expressed by macrophages, neutrophils and epithelial cells; it has neutrophil chemoattractant activity. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection and may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. | ||