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Catalog Number:VQ1839M
Mouse MMP-3 VeriQuant Immunoassay Kit
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96 T

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(a) Two monoclonal antibodies against distinct epitopes are conjugated with oligo probes, then form a sandwich complex with the antigen. (b) Following antigen recognition by the paired antibodies, the conjugated oligonucleotides are spatially proximate, and generate an amplifiable dsDNA template under the enzymatic action. (c) qPCR amplification is then performed. CT values correlate inversely with protein concentration, and sample target concentrations are calculated via standard curve.
Color-coded reagents components for easy identification
Kit outer packaging and components packaging
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Product Details
Product Name Mouse MMP-3 VeriQuant Immunoassay Kit Species Mouse
Gene Alias Mmp3 / MMP-3 / SL-1 / EMS-2 Gene ID 17392
Uniprot ID P28862 Protein Alias Mouse MMP-3
Detection Method Proximity-based Immunoassay Sensitivity 0.978pg/mL
Assay Instrument qPCR Instruments Detection Range 3.2-50000 pg/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids;Sample Volume=1~5μL
Total Assay Time 2 H Manual Handling Time 20 min
Shipping Temperature Ultra-low temperature phase-change materials Storage Temperature -20°C
No Washing Step Yes Tag or Dye FAM™
Kit Info N/A
Target Info [Note: Novoprotein does not offer a mouse MMP-3 product; the text below is taken from the Human MMP-3 product (C378).] MMP3 is a member of the matrix metalloproteinase (MMP) family whose members are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes including arthritis and metastasis. The MMP-3 enzyme degrades collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP-3 can also activate other MMPs such as MMP-1, MMP-7, and MMP-9, rendering MMP-3 crucial in connective tissue remodeling.[3] The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.